I was there in my sisters' room playing MRDS while I was recording approximately 6 hours worth of crap on my DVD Recorder due to some experimentation I need to do. Since the channel was already on CN, I just recorded it there (might find something interesting to watch though, unfortunately no). On the 3rd hour, this Chowder episode came on. I don't know much about what happened since I wasn't paying attention, when I suddenly heard this line out of nowhere. I suddenly looked at the TV for a while, and found it weird that I was hearing it from one of the more random English cartoons. Then I searched for it on the net and found that the line was really used here.
I don't really play Portal so I wouldn't know anything about this line either. Just found it amusing how a meme found its way into a cartoon that I never even watched.